GLDH (Glutamate Dehydrogenase) calibrators are reference materials used in laboratory assays to calibrate the measurement of GLDH enzyme activity. GLDH is an enzyme found in various tissues, particularly in the liver and kidneys, and it plays a crucial role in amino acid metabolism. Elevated levels of GLDH in the blood can indicate liver or kidney damage, making GLDH assays valuable in diagnosing liver and kidney diseases.
GLDH calibrators typically consist of solutions containing known concentrations of GLDH enzyme activity. These calibrators are used to establish a standard curve in GLDH assays, allowing for the accurate quantification of GLDH activity in samples. By measuring the absorbance or fluorescence produced by GLDH-mediated reactions with specific substrates, the enzyme activity in samples can be determined relative to the calibrator concentrations.
In actual laboratory practice, GLDH calibrators are essential for ensuring the accuracy and reliability of GLDH assays. They help validate assay performance, verify instrument calibration, and enable the quantification of GLDH enzyme activity within a defined range. Quality control materials, including GLDH calibrators, are crucial components of diagnostic assays, providing confidence in the accuracy of test results.